Direct somatic embroygenesis from immature embryos of rosewood (Dalbergia latifolia Roxb.)

Rao, M Muralidhar and Sita, G Lakshmi (1996) Direct somatic embroygenesis from immature embryos of rosewood (Dalbergia latifolia Roxb.). In: Plant Cell Reports, 15 (5). 355 -359.

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Abstract

Direct regeneration of somatic embryos was obtained from immature zygotic embryos of Dalbergia latifolia. Immature embryos dissected from green pods 90 d after flowering gave the highest frequency of somatic embryo formation. Preculture on high 2,4-D medium for 4 weeks induced direct somatic embryogenesis, which was expressed during the second culture phase in the presence of low 2,4-D along with a high sucrose concentration. Embryos were separated and transferred to the maturation medium containing MS + 0.5-1.0 mg/L BAP, where embryos developed into plantlets. Somatic embryos failed to convert into complete plants without BAP treatment. This method of direct regeneration of somatic embryos without a callus phase has direct application for genetic manipulation studies.

Item Type:	Journal Article
Publication:	Plant Cell Reports
Publisher:	Springer
Additional Information:	copyright of this article belongs to Springer.
Keywords:	Plant-regeneration;clonal propagation;zygotic embryos;invitro;tissue;callus.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	18 Jan 2010 09:11
Last Modified:	19 Sep 2010 05:53
URI:	http://eprints.iisc.ac.in/id/eprint/25094

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