Expression of glucose transporter isoforms and the insulin receptor during hamster preimplantation embryo development

Tonack, Sarah and Ramin, Nicole and Garimella, Sireesha and Rao, Rajnish and Seshagiri, Polani B and Fischer, Bernd and Santos, Anne Navarrete (2009) Expression of glucose transporter isoforms and the insulin receptor during hamster preimplantation embryo development. In: Annals Of Anatomy-Anatomischer Anzeiger, 191 (5). pp. 485-495.

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Abstract

During preimplantation development, embryos of many species are known to express up to five isoforms of the facilitative glucose transporter proteins (GLUT). Development of hamster blastocysts is inhibited by glucose. We therefore investigated GLUT isoform and insulin receptor (IR) expression in hamster preimplantation embryos cultured in glucose-free medium from the 8-cell stage onwards. We show that GLUT1, 3 and 8 mRNA are constitutively expressed from the 8-cell to the blastocyst stage. The IR is expressed from the morula stage onwards. Messenger RNA of the insulin-responsive GLUT4 was not detected at any stage. GLUT1 and 3 were localised by immunocytochemistry. GLUT1 was expressed in both embryoblast and trophoblast, in the latter, mainly in basal and lateral membranes directed towards the blastocoel. and embryoblast. GLUT3 was exclusively localised in the apical. membrane of trophoblast cells. We show that hamster preimplantation embryos express several GLUT isoforms thus closely resembling embryos of other mammalian species. Despite endogenous IR expression, the insulin-sensitive isoform GLUT4 was not expressed, indicating that the insulin-mediated glucose uptake known from classical insulin target cells may not be relevant for hamster blastocysts.

Item Type:	Journal Article
Publication:	Annals Of Anatomy-Anatomischer Anzeiger
Publisher:	Elsevier Science
Additional Information:	Copyright for this article belongs to Elsevier Science.
Keywords:	GLUT; Insulin receptor; Mammalian preimplantation development; Golden hamster; Glucose transport
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	07 Dec 2009 09:56
Last Modified:	19 Sep 2010 05:52
URI:	http://eprints.iisc.ac.in/id/eprint/24964

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