Sreenagesh, MV and Jayabaskaran, C (1996) Light- and cytokinin-induced changes in the levels of leucine and tyrosine isoaccepting tRNA species and modified nucleotide contents of total tRNA in cucumber seedlings. In: Biochemistry & Molecular Biology International, 38 (6). pp. 1255-1264.
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We have determined relative levels of chloroplast leucine and tyrosine isoaccepting tRNAs and modified nucleotide contents from total tRNAs isolated from dark-grown, light-grown, N6-isopentenyladenine (i6A)-treated dark-grown and i6A-treated light-grown cucumber seedlings. Significant increases in the relative amounts of tRNA(Leu)2 and tRNA(Leu)3 were observed in the i6A-treated dark-grown seedlings compared to dark-grown, light-grown and i6A-treated light-grown seedlings. On the other hand, i6A-treated light-grown seedlings tRNA(Tyr)1 increased to 85% of total tRNAs(Tyr) from about 9% in light-grown seedlings and tRNA(Tyr)2 decreased to 15% compared with 91% in light-grown seedlings. Analysis of modified nucleotide of total tRNAs indicated that pT, pI, pm1A, pm5C, pGm, pm1G, pm2G and pm7G contents were significantly higher in the total tRNA of i6A-treated dark-grown seedlings than those from untreated dark-grown seedlings. Illumination of 8-day-old dark-grown seedlings for 12 h increased the contents of pT, pI, pGm and pm1G when compared to 8-day-old dark-grown seedlings with extended growth for 12 h in dark. On the contrary, i6A had no stimulatory effect in the contents of modified nucleotide in the light-grown seedlings.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry & Molecular Biology International |
| Publisher: | Academic press aust |
| Additional Information: | Copyright of this article belongs to Academic press aust. |
| Keywords: | Gene-expression;chloroplast development;phaseolus-vulgaris;transfer-rnas. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 20 Jan 2010 10:56 |
| Last Modified: | 20 Jan 2010 10:56 |
| URI: | http://eprints.iisc.ac.in/id/eprint/24779 |
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