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Inhibition of replication of rinderpest virus by 5-fluorouracil

Ghosh, Arundhati and Nayak, Rabindranath and Shaila, MS (1996) Inhibition of replication of rinderpest virus by 5-fluorouracil. In: Antiviral Research, 31 (1-2). pp. 35-44.

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5-Fluorouracil (5FU), an analogue of uracil, was found to inhibit the production of infectious particles of rinderpest virus (RPV) in Vero cells (African green monkey kidney cells) by 99%, at a concentration of 1 μg/ml. The levels of individual mRNA specific for five of the virus genes were also reduced drastically, while the level of mRNA for a cellular housekeeping gene—glyceraldehyde-3-phosphate dehydrogenase (GAPDH)—was unaltered by fluorouracil treatment of infected cells. Both virus RNA and protein synthesis showed inhibition in a dose-dependent manner. The virions which budded out of 5-fluorouracil-treated cells also contained reduced amounts of virus proteins compared with virus particles from untreated cells.

Item Type: Journal Article
Publication: Antiviral Research
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Rinderpest virus;5-Fluorouracil;Virus transcription; Antiviral drug.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 21 Jan 2010 05:32
Last Modified: 19 Sep 2010 05:51
URI: http://eprints.iisc.ac.in/id/eprint/24770

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