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A simple method for large-scale preparation of chick embryo tRNA

Anandaraj, MP Jacob Spurgeon and Cherayil, Joseph D (1974) A simple method for large-scale preparation of chick embryo tRNA. In: Analytical Biochemistry, 58 (1). pp. 190-194.

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A simple method for preparing bulk quantities of tRNA from chick embryo has been developed. In this method chick embryos were homogenized in a buffer of pH 4.5, followed by deproteinization with phenol. The aqueous layer was allowed to separate under gravity. The resulting aqueous layer, after two more phenol treatments, was directly passed through a DEAE-cellulose column and the tRNA eluted therefrom with 1 Image NaCl. The tRNA prepared by this method was as active as the one prepared at neutral pH.

Item Type: Journal Article
Publication: Analytical Biochemistry
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 31 Dec 2009 09:13
Last Modified: 19 Sep 2010 05:50
URI: http://eprints.iisc.ac.in/id/eprint/24421

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