Two forms of methionyl-transfer RNA synthetase from Mycobacterium Smegmatis

Deobagkar, Dilip N and Gopinathan, KP (1976) Two forms of methionyl-transfer RNA synthetase from Mycobacterium Smegmatis. In: Biochemical and Biophysical Research Communications, 71 (4). pp. 939-951.

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Abstract

Two methionyl-transfer RNA synthetases (A and B forms) have been isolated from Image . The homogeneous preparations of the enzymes showed 1500 fold increase in specific activity in aminoacylation of methionine specific tRNA. The A and B forms differed in their specificity of aminoacylation of tRNAmMet and tRNAfMet; enzyme B exhibited much higher specificity for tRNAfMet. The molecular activities of A and B enzymes for aminoacid and tRNA were identical. The turnover number for aminoacid was 27 fold greater than that for tRNA, while the Km values for tRNA were lower by a factor of 106 as compared to the aminoacid. Both the enzymes catalysed ATP-pyrophosphate exchange reaction to the same extent.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elservier sciecne
Additional Information:	Copyright of this article belongs to Elservier sciecne
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	09 Dec 2009 12:02
Last Modified:	19 Sep 2010 05:49
URI:	http://eprints.iisc.ac.in/id/eprint/24258

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