Kranthi, Balla Venkata and Kumar, Ritesh and Kumar, Nallani Vijay and Rao, Desirazu N and Rangarajan, Pundi N (2009) Identification of key DNA elements involved in promoter recognition by Mxr1p, a master regulator of methanol utilization pathway in Pichia pastoris. In: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1789 (6-8). pp. 460-468.
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Abstract
Mxr1p (methanol expression regulator 1) functions as a key regulator of methanol metabolism in the methylotrophic yeast Pichia pastoris. In this study, a recombinant Mxr1p protein containing the N-terminal zinc finger DNA binding domain was overexpressed and purified from E coli cells and its ability to bind to promoter sequences of AOXI encoding alcohol oxidase was examined. In the AOXI promoter, Mxr1p binds at six different regions. Deletions encompassing these regions result in a significant decrease in AOXI promoter activity in vivo. Based on the analysis of AOXI promoter sequences, a consensus sequence for Mxr1p binding consisting of a core 5' CYCC 3' motif was identified. When the core CYCC sequence is mutated to CYCA, CYCT or CYCM (M = 5-methylcytosine), Mxr1p binding is abolished. Though Mxr1p is the homologue of Saccharomyces cerevisiae Adr1p transcription factor, it does not bind to Adr1p binding site of S. cerevisiae alcohol dehydrogenase promoter (ADH2UAS1). However, two point mutations convert ADH2UAS1 into an Mxr1p binding site. The identification of key DNA elements involved in promoter recognition by Mxr1p is an important step in understanding its function as a master regulator of the methanol utilization pathway in P. pastoris.
Item Type: | Journal Article |
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Publication: | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms |
Publisher: | Elsevier B.V. |
Additional Information: | Copyright of this article belongs to Elsevier B. V., 2009. |
Keywords: | Alcohol oxidase; Pichia pastoris; Zinc finger protein; Methanol; Transcription; DNase I foot printing |
Department/Centre: | Division of Biological Sciences > Biochemistry |
Date Deposited: | 17 Dec 2009 12:06 |
Last Modified: | 19 Sep 2010 05:49 |
URI: | http://eprints.iisc.ac.in/id/eprint/24230 |
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