ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Oxidation of succinate in heart, brain, and kidney mitochondria in hypobaria and hypoxia

Sivaramakrishnan, S and Ramasarma, T (1975) Oxidation of succinate in heart, brain, and kidney mitochondria in hypobaria and hypoxia. In: Environmental Physiology & Biochemistry, 5 (3). 189 -200.

Full text not available from this repository. (Request a copy)
Official URL: http://www.ncbi.nlm.nih.gov/pubmed/168066


Exposure of rats to hypobaric stress for periods of up to 36 h caused a consistent change in the succinate-NT reductase activity of the heart mitochondria whereas there was no significant change in the activities of either succinate dehydrogenase and succinate-NT reductase of the brain and the kidney. Mitochondrial succinate dehydrogenase of the heart, the brain and the kidney was activated 2- to 7-fold with the substrate and malonate. The activations obtained with oxalate, citrate and dinitrophenol were relatively lower in comparison to succinate and malonate. Benzohydroquinone and 2-nitrophenol had no stimulatory effect on the heart, the brain and the kidney mitochondria. THE ACTIVATIONS OBTAINED WITH THE VARIOUS EFFECTORS PARTIALLY (OR COMPLETELY IN THE CASE OF SUCCINATE) REVERSED ON WASHING THE MITOCHONDRIAL SAMPLES WITH THE SUCROSE HOMOGENIZING MEDIUM. The effect of ubiquinol, which also activated the enzyme, was only partially reversed after the second preincubation with succinate in the brain and the kidney whereas in the heart the activity was fully reversed. The increased activity of succinate dehydrogenase obtained with ATP and ADP was further enhanced by Mg2+ exclusively in the brain mitochondria, suggesting the possibility of Mg2+-AIP complex as the active species. Succinate-NT reductase of the heart, the brain and the kidney mitochondria showed a high activation with ubiquinone whereas its reduced form had no stimulatory effect.

Item Type: Journal Article
Publication: Environmental Physiology & Biochemistry
Publisher: Munksgaard int publ ltd
Additional Information: Copyright of this article belongs to Munksgaard int publ ltd.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 23 Jan 2010 04:00
Last Modified: 23 Jan 2010 04:00
URI: http://eprints.iisc.ac.in/id/eprint/24043

Actions (login required)

View Item View Item