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Mammalian brain plasma membranes: Isolation, enzymatic, and chemical characterization

Krishnan, KS and Balaram, P (1976) Mammalian brain plasma membranes: Isolation, enzymatic, and chemical characterization. In: Experimental Cell Research, 101 (2). pp. 299-306.

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Two variants of a simplified procedure for the isolation of plasma membrane fractions from monkey and rat brains, are described. The preparations show marked enrichments in the marker enzymes, (Na+-K+) adenosine triphosphatase, acetylcholinesterase, 5′-nucleotidase and adenylate cyclase. Lipid analysis and a protein electrophoretic pattern are presented. An enzymatic check has been made to assess for contamination by other cellular organelles. The amino acid composition of brain membrane proteins show a resemblance to the reported composition of erythrocyte ghost proteins but differ from myelin proteins.

Item Type: Journal Article
Publication: Experimental Cell Research
Publisher: Elservier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 11 Dec 2009 06:17
Last Modified: 19 Sep 2010 05:48
URI: http://eprints.iisc.ac.in/id/eprint/24041

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