Studies on Rat Ovarian Receptors for Lutropin (Luteinizing Hormone): Applicability of radioimmunoassay to measure lutropin bound to receptors.

Muralidhar, Kambadur and Modugal, N Raghuveer (1977) Studies on Rat Ovarian Receptors for Lutropin (Luteinizing Hormone): Applicability of radioimmunoassay to measure lutropin bound to receptors. In: Biochemical Journal, 160 (3). pp. 603-607.

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Abstract

Measurement of receptor-bound unlabelled physiologically active lutropin (luteinizing hormone, LH) was possible by a modified radioimmunoassay. The conventional radioimmunoassayconducted at 4°C was inadequate, whereas the modified assay performed at 37'C could measure receptor-bound lutropin. The radioimmunoassay at 37'C takes only 36h for completion compared with 5-7 days at 4°C. The sensitivity and range of dose-response curves are, however, unaltered. The validity of the technique was established by a number of criteria.

Item Type:	Journal Article
Publication:	Biochemical Journal
Publisher:	Portland Press
Additional Information:	Copyright of this article belongs to Portland Press.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	13 Nov 2009 11:26
Last Modified:	19 Sep 2010 05:47
URI:	http://eprints.iisc.ac.in/id/eprint/23861

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