Purification and Characterization of Restriction Endonuclease Basl from Bacillus species

Chandrashekaran, Siddamadappa and Babu, Padmanabhan and Nagaraja, Valakunja (1999) Purification and Characterization of Restriction Endonuclease Basl from Bacillus species. In: Journal Biochemistry, Molecular Biolology & Biophysics, 3 . pp. 225-229.

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Abstract

After screening a variety of bacteria from soil for the DNA cleavage activity, a type II restriction endonuclease, Basl was isolated and characterized from a Bacillus species. Using the purified enzyme the recognition sequence was determined. The enzyme recognizes 5'- CCANNNN NTGG-3' sequence and cleaves as indicated. The enzyme functions efficiently at low ionic strength, narrow pH range and at the optimum temperature of 30°C.

Item Type:	Journal Article
Publication:	Journal Biochemistry, Molecular Biolology & Biophysics
Publisher:	Overseas Publishers Association
Additional Information:	Copyright for this article belongs to Gordon and Breach Publishing Group.
Keywords:	Restriction endonuclease;Bacillus species;lsoschizomer
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	08 Dec 2004
Last Modified:	19 Sep 2010 04:17
URI:	http://eprints.iisc.ac.in/id/eprint/2378

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