Evidence for the involvement of a rapidly turning over protease in the degradation of cytochrome oxidase in Neurospora crassa

Padmanaban, CG and Padmanaban, G (1981) Evidence for the involvement of a rapidly turning over protease in the degradation of cytochrome oxidase in Neurospora crassa. In: Biochemical and Biophysical Research Communications, 100 (2). pp. 576-583.

PDF full_text_1.pdf - Published Version Restricted to Registered users only Download (291kB) | Request a copy

Abstract

Treatment of N. crassa cultures with cycloheximide followed by washing and incubation in drug-free fresh medium results in a rapid decline in cytochrome oxidase activity. This is associated with the degradation of higher molecular weight subunits of cytochrome oxidase under these conditions. The protease activity associated with the mitochondrial preparation decreases during cycloheximide treatment and rapidly returns to normal levels on subsequent washing and transfer to drug-free fresh medium. It is suggested that the steady-state level of cytochrome oxidase is governed by a rapidly turning over cytoplasmically synthesized mitochondrial protease.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Inc.
Additional Information:	Copyright of this article belongs to Elsevier Inc.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	05 Jan 2010 06:13
Last Modified:	19 Sep 2010 05:46
URI:	http://eprints.iisc.ac.in/id/eprint/23603

Actions (login required)

View Item