Vanadate-stimulated NADH oxidation in plasma membrane

Ramasarma, T and MacKellar, WC and Crane, FL (1981) Vanadate-stimulated NADH oxidation in plasma membrane. In: Biochimica et Biophysica Acta (BBA) - Biomembranes, 646 (1). pp. 88-98.

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Abstract

The rate of NADH oxidation with oxygen as the acceptor is very low in mouse liver plasma membrane and erythrocyte membrane. When vanadate is added, this rate is stimulated 10- to 20-fold. The absorption spectrum of vanadate does not change with the disappearance of NADH. The reaction is inhibited by superoxide dismutase, and there is no activity under an argon atmosphere. This indicates that oxygen is the electron acceptor and the reaction is mediated by superoxide. The vanadate stimulation is not limited to plasma membrane. Golgi apparatus and endoplasmic reticulum show similar increase in NADH oxidase activity when vanadate is added. The endomembranes have significant vanadate-stimulated activity with both NADH and NADPH. The vanadate-stimulated NADH oxidase in plasma membrane is inhibited by compounds, which inhibit NADH dehydrogenase activity: catechols, anthracycline drugs and manganese. This activity is stimulated by high phosphate and sulfate anion concentrations.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher:	Elsevier B.V.
Additional Information:	Copyright of this article belongs to Elsevier B.V.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	20 Jan 2010 07:30
Last Modified:	19 Sep 2010 05:46
URI:	http://eprints.iisc.ac.in/id/eprint/23495

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