ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Number and proportion of selenocucleosides in the transfer RNA of escherichia-coli

Rao, YS Prasada and Cherayil, Joseph D (1974) Number and proportion of selenocucleosides in the transfer RNA of escherichia-coli. In: Life Sciences, 14 (10). pp. 2051-2059.

[img] PDF
fullpdf.pdf - Published Version
Restricted to Registered users only

Download (316kB) | Request a copy
Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...


tRNA isolated from escherichia-coli grown in a medium containing [75Se] sodium selenosulfate was converted to nucleosides and analysed for selenonucleosides on a phosphocellulose column. Upon chromatography of the nucleosides on phosphocellulose column, the radioactivity resolved into three peaks. The first peak consisted of free selenium and traces of undigested nucleotides. The second peak was identified as 4-selenouridine by co-chromatographing with an authentic sample of 4-selenouridine. The identity of the third peak was not established. The second and third peaks represented 93% and 7% of the selenium present in nucleosides respectively.

Item Type: Journal Article
Publication: Life Sciences
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 19 Dec 2009 07:43
Last Modified: 19 Sep 2010 05:45
URI: http://eprints.iisc.ac.in/id/eprint/23296

Actions (login required)

View Item View Item