ESR study of metal ion-guanosine monophosphate interaction

Podder, SK and Rengan, S Kasthuri and Navalgund, Ranganath (1974) ESR study of metal ion-guanosine monophosphate interaction. In: Journal of Magnetic Resonance, 15 (2). pp. 254-261.

PDF text.pdf - Published Version Restricted to Registered users only Download (432kB) | Request a copy

Abstract

The addition of guanosine 5-monophosphate (5′-GMP) to an aqueous solution of Mn2+ ions results in a decrease in ESR signal intensity and an increase in line-width of Mn2+ ions. This can be interpreted in terms of stepwise formation of outersphere and inner-sphere complexes as When Mg2+ is added to a mixture of Mn2+ and 5′-GMP, ESR signal intensity increases, presumably due to the replacement of Mn2+ by Mg2+ in the complex. From the variation of ESR signal intensity as a function of concentration of Mg2+, the product K1K2 for the magnesium complex i s calculated as 125 M−1. This difference in stability constants may indicate that both phosphate group and guanine base are involved in the formation of Mn2+-5′-GMP complex.

Item Type:	Journal Article
Publication:	Journal of Magnetic Resonance
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	21 Dec 2009 10:32
Last Modified:	19 Sep 2010 05:44
URI:	http://eprints.iisc.ac.in/id/eprint/23164

Actions (login required)

View Item