Studies on the effects of in vitro methyiation on aminoacylation of transfer RNA

Vani, BR and Ramakrishnan, TV (1984) Studies on the effects of in vitro methyiation on aminoacylation of transfer RNA. In: Journal of Biosciences, 6 (2). pp. 213-220.

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Abstract

In vitro methyiation of Escherichia coli transfer ribonucleic acid by cell free extracts of Mycobacterium smegmatis leads exclusively to the formation of 1-methyl adenine [Vani, B. R., Ramakrishnan, T., Taya, Y., Noguchi, S., Yamaiuzumi, Z. and Nishimura, S.(1978) J. Bact., 137,1085]. We have studied the effect of this modification on aminoacylation of Escherichia coli tRNA by mycobacterial enzymes. Aminoacylation with total algal protein hydrolysate as well as several individual aminoacids like methionine, valine, tyrosine, aspartic acid and lysine were monitored. In all the cases methyiation had a positive effect on the extent of aminoacylation by mycobacterial enzymes. Decreased aminoacylation in vitro was observed when hypomethylated transfer RNA from ethionine treated cells was used as the substrate for aminoacylation.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian academy of sciences
Additional Information:	Copyright for this article belongs to Indian academy of sciences.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	22 Jan 2010 05:49
Last Modified:	19 Sep 2010 05:43
URI:	http://eprints.iisc.ac.in/id/eprint/22835

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