Separation of 5-methylcytosine-rich DNA using immobilized antibody

Deobagkar, DN and Shankar, V and Deobagkar, DD (1986) Separation of 5-methylcytosine-rich DNA using immobilized antibody. In: Enzyme and Microbial Technology, 8 (2). pp. 97-100.

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Abstract

Anti-(5-methylcytosine) antibodies were immobilized on glutaraldehyde-activated Indion 48-R, a polystyrene resin with amino groups. Immobilized antibody was very stable and could be used several times without any apparent change in the initial binding capacity of the antibody-matrix. Fractions of total DNA from various animal and plant sources were retained on this column and could be eluted quantitatively with 1.0 m NaCl. The bound fraction was further characterized for its 5-methylcytosine content by restriction enzyme digestion patterns.

Item Type:	Journal Article
Publication:	Enzyme and Microbial Technology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	27 Jan 2010 07:03
Last Modified:	19 Sep 2010 05:43
URI:	http://eprints.iisc.ac.in/id/eprint/22725

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