Deobagkar, DN and Shankar, V and Deobagkar, DD (1986) Separation of 5-methylcytosine-rich DNA using immobilized antibody. In: Enzyme and Microbial Technology, 8 (2). pp. 97-100.
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Abstract
Anti-(5-methylcytosine) antibodies were immobilized on glutaraldehyde-activated Indion 48-R, a polystyrene resin with amino groups. Immobilized antibody was very stable and could be used several times without any apparent change in the initial binding capacity of the antibody-matrix. Fractions of total DNA from various animal and plant sources were retained on this column and could be eluted quantitatively with 1.0 m NaCl. The bound fraction was further characterized for its 5-methylcytosine content by restriction enzyme digestion patterns.
Item Type: | Journal Article |
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Publication: | Enzyme and Microbial Technology |
Publisher: | Elsevier Science |
Additional Information: | Copyright of this article belongs to Elsevier Science. |
Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 27 Jan 2010 07:03 |
Last Modified: | 19 Sep 2010 05:43 |
URI: | http://eprints.iisc.ac.in/id/eprint/22725 |
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