Molecular analysis of inorganic nitrogen assimilation in yeasts

Choudary, Prabhakara V and Rao, G Ramananda (1984) Molecular analysis of inorganic nitrogen assimilation in yeasts. In: Archives of Microbiology, 138, N (3). p. 183.

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Abstract

Assimilation of nitrate and various other inorganic nitrogen compounds by different yeasts was investigated. Nitrate, nitrite, hydroxylamine, hydrazine, ammonium sulphate, urea and L-asparagine were tested as sole sources of nitrogen for the growth of Candida albicans, C. pelliculosa, Debaryomyces hansenii, Saccharomyces cerevisiae, C. tropicalis, and C. utilis. Ammonium sulphate and L-asparagine supported the growth of all the yeasts tested except D. hansenii while hydroxylamine and hydrazine failed to support the growth of any. Nitrate and nitrite were assimilated only by C. utilis. Nitrate utilization by C. utilis was also accompanied by the enzymatic activities of NAD(P)H: nitrate oxidoreductase (EC 1.6.6.2) and NAD(P)H: nitrite oxidoreductase (EC 1.6.6.4), but not reduced methyl viologen-or FAD-nitrate oxidoreductases (EC 1.7.99.4). It is demonstrated here that nitrate and nitrite reductase activities are responsible for the ability of C. utilis to assimilate primary nitrogen.

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer
Additional Information:	Copyright for this article belongs to Springer.
Keywords:	Yeast; Nitrogen assimilation; Nitrate reductase; Nitrite reductase; Candida utilis; Food yeast; Nitrate reduction; Nitrogenous oxide
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	22 Jan 2010 07:36
Last Modified:	19 Sep 2010 05:42
URI:	http://eprints.iisc.ac.in/id/eprint/22671

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