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Alterations in the activities of the enzymes of proline metabolism in Ragi (Eleusine coracana) leaves during water stress

Kandpal, Rajendra P and Vaidyanathan, CS and Kumar, Udaya M and Sastry, Krishna KS and Rao, Appaji N (1981) Alterations in the activities of the enzymes of proline metabolism in Ragi (Eleusine coracana) leaves during water stress. In: Journal of Biosciences, 3 (4). pp. 361-370.

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Free proline content in Ragi (Eleusine coracana) leaves increased markedly (6 to 85 fold) as the degree of water stress, created by polyethylene gylcol treatment, was prolonged There was also a marginal increase in soluble proteins in the stressed leaves as compared to that in the controls. Water stress stimulated the activities of ornithine aminotransferase and pyrroline-5-carboxylate reductase, the enzymes of proline biosynthesis and markedly inhibited the enzymes involved in proline degradation viz., proline oxidase and pyrroline-5-carboxylate dehydrogenase. These results suggest that increase in free proline content of Ragi leaves could be due to enhanced activities of the enzymes synthesizing proline but more importantly due to severe inhibition of the enzymes degrading proline. These observations establish for the first time, the pathway of proline metabolism in plants by way of detection of the activities of all the enzymes involved and also highlight the role of these enzymes in proline accumulation during water stress.

Item Type: Journal Article
Publication: Journal of Biosciences
Publisher: Indian Academy of Sciences
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: Proline;water stress;Eleusine coracana;ornithine aminotransferase;pyrroline-5-carboxylate reductase;proline oxidase;pyrroline-5-carboxylate dehydrogenase
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 27 Aug 2009 10:37
Last Modified: 19 Sep 2010 05:42
URI: http://eprints.iisc.ac.in/id/eprint/22590

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