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Chemical modification of Methionines of Ribonuclease a with O-Benzoquinone

Gupta, Munishwar N and Vithayathil, Paul J (1980) Chemical modification of Methionines of Ribonuclease a with O-Benzoquinone. In: International Journal of Peptide and Protein Research, 15 (3). pp. 236-242.

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Official URL: http://www3.interscience.wiley.com/journal/1215307...


The accessibility of methionines in RNAase A to reaction with OBQ has been studied at highly acidic pH. The differences between the rate constants of reactions of the methionine and methionines of RNAase A with OBQ is a reflection on the limited accessibility of methionines in the protein conformation. Nevertheless, at sufficiently high OBQ concentration, all the four methionines of the enzyme can be modified. At lower concentration of OBQ, a derivative may be prepared in which a specific methionine is modified. The introduced chromophore ionizes at around pH 3 in this derivative. The derivative has partial activity towards RNA which is enhanced on addition of S-protein.

Item Type: Journal Article
Publication: International Journal of Peptide and Protein Research
Publisher: John Wiley and Sons
Additional Information: Copyright of this article belongs to John Wiley and Sons.
Keywords: Abnormal ionization;chemical modification of methionines; o-benzoquinone;ribonuclease A.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 26 Aug 2009 12:23
Last Modified: 26 Aug 2009 12:23
URI: http://eprints.iisc.ac.in/id/eprint/22522

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