ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Occurrence, divergence and evolution of intrinsic terminators across Eubacteria

Mitra, Anirban and Angamuthu, Kandavelmani and Jayashree, Hanasoge Vasudevamurthy and Nagaraja, Valakunja (2009) Occurrence, divergence and evolution of intrinsic terminators across Eubacteria. In: Genomics, 94 (2). pp. 110-116.

[img] PDF
118.pdf - Published Version
Restricted to Registered users only

Download (498kB) | Request a copy
Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...


In Escherichia coli, the canonical intrinsic terminator of transcription includes a palindrome followed by a U-trail on the transcript. The apparent underrepresentation of such terminators in eubacterial genomes led us to develop a rapid and accurate algorithm, GeSTer, to predict putative intrinsic terminators. Now, we have analyzed 378 genome sequences with an improved version of GeSTer. Our results indicate that the canonical E. coli type terminators are not overwhelmingly abundant in eubacteria. The atypical structures, having stem-loop structures but lacking ‘U’ trail, occur downstream of genes in all the analyzed genomes but different phyla show conserved preference for different types of terminators. This propensity correlates with genomic GC content and presence of the factor, Rho. 60–70% of identified terminators in all the genomes show “optimized” stem-length and ΔG. These results provide evidence that eubacteria extensively rely on the mechanism of intrinsic termination, with a considerable divergence in their structure, positioning and prevalence. The software and detailed results for individual genomes are freely available on request

Item Type: Journal Article
Publication: Genomics
Publisher: Elsevier Science
Additional Information: copyright of this article belongs to Elsevier Science.
Keywords: Transcription; Intrinsic termination; GeSTer; Eubacteria; Palindrome; Stem-loop; Rho
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 23 Dec 2009 09:28
Last Modified: 19 Sep 2010 05:41
URI: http://eprints.iisc.ac.in/id/eprint/22274

Actions (login required)

View Item View Item