A simple procedure for purification of N-carbamoylputrescine: Application to assays of putrescine transcarbamoylase and agmatine iminohydrolase activities

Venugopal, Sri KS and Adiga, PR (1980) A simple procedure for purification of N-carbamoylputrescine: Application to assays of putrescine transcarbamoylase and agmatine iminohydrolase activities. In: Analytical Biochemistry, 104 (2). pp. 440-444.

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Abstract

The observation that N-carbamoylputrescine is quantitatively excluded on O-(carboxymethyl)-cellulose columns with simultaneous retention of putrescine and agmatine has been utilized to develop a sensitive radiometric assay for putrescine transcarbamoylase and a colorimetric assay for agmatine iminohydrolase. A simple procedure for obtaining bulk amounts of pure synthetic N-carbamoylputrescine by separation from putrescine and dicarbamoylputrescine on Dowex 50 (Na+) resin is described.

Item Type:	Journal Article
Publication:	Analytical Biochemistry
Publisher:	Elsevier science
Additional Information:	Copyright of this article belongs to Elsevier science.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	27 Jan 2010 11:50
Last Modified:	01 Mar 2012 06:37
URI:	http://eprints.iisc.ac.in/id/eprint/22015

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