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Estrogen Induction Of Biotin-Binding Protein In Immature Chicks - Kinetics, Hormonal Specificity And Modulation

Murty, Ramana CV and Adiga, Radhakantha P (1985) Estrogen Induction Of Biotin-Binding Protein In Immature Chicks - Kinetics, Hormonal Specificity And Modulation. In: Molecular and Cellular Endocrinology, 40 (1). pp. 79-86.

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Official URL: http://dx.doi.org/10.1016/0303-7207(85)90160-1


Employing a specific radioimmunoassay for quantification, the kinetics of estrogen-induced elevation in the plasma concentration of biotin-binding protein (BBP) in immature male chicks was investigated. A single injection of the steroid hormone enhanced the plasma BBP content several-fold at 6 h, reaching peak levels around 48 h and declining thereafter. A 2-fold amplification of the response was evident during secondary stimulation with the hormone. The magnitude of the response was hormonal dose-dependent while the initial lag phase and the time of peak protein accumulation were unaltered within the hormonal doses tested. The circulatory half-life of the specific protein in normal and estrogenized birds was 10 h. Hyperthyroidism markedly decreased the hormonal response while the opposite effect was seen during hypothyroidism. The antiestrogens E- and Z-clomiphene citrate effectively blocked the protein induction whereas progesterone, either alone or in combination with estrogen, was ineffective in modulating the induction. Cycloheximide administration drastically inhibited the inductive response. The above observations clearly suggest that the genes corresponding to the two isofunctional proteins of chicken egg, viz. BBP and avidin, are differentially regulated.

Item Type: Journal Article
Publication: Molecular and Cellular Endocrinology
Publisher: Elsevier Science
Additional Information: The Copyright of this article belongs to Elsevier Science.
Keywords: radioimmunoassay;thyroid status;gene expression; antiestrogens;progesterone;cycloheximide.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 11 Aug 2009 08:34
Last Modified: 01 Mar 2012 07:45
URI: http://eprints.iisc.ac.in/id/eprint/21877

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