Purification of clofibrate-induced carnitine acetyltransferase from rat liver mitochondria

Mittal, Balraj and Kurup, CK Ramakrishna (1980) Purification of clofibrate-induced carnitine acetyltransferase from rat liver mitochondria. In: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 619 (1). pp. 90-97.

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Abstract

The enzyme carnitine acetyltransferase (acetyl-CoA:carnitine O-acetyltransferase, EC 2.3.1.7) has been purified to homogeneity from hepatic mitochondria of clofibrate-fed rats. It is a protein of molecular weight 56 000 composed of two non-identical subunits of molecular weight 34 000 and 25 000. The enzyme is inhibited by palmityl-CoA as well as acetyl carnitine. The inhibition by fatty acyl-CoA is competitive with respect to both the substrates, carnitine and acetyl-CoA. The inhibition by acetylcarnitine is reversed by carnitine but not by acetyl-CoA.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Publisher:	Elsevier Science B.V.
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Clofibrate induction; Carnitine acetyltransferase; (Rat liver mitochondria)
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	28 Jan 2010 09:46
Last Modified:	19 Sep 2010 05:38
URI:	http://eprints.iisc.ac.in/id/eprint/21610

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