Influence of starvation and clofibrate administration on oxidative phosphorylation by rat liver mitochondria

Rasheed, BK and Chhabra, S and Kurup, CK (1980) Influence of starvation and clofibrate administration on oxidative phosphorylation by rat liver mitochondria. In: Biochemical Journal, 190 (1). pp. 191-198.

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Abstract

Whole cells, homogenates and mitochondrial obtained from the livers of albino rats which were starved for 6 days or more showed a 50% decrease in oxidative activity. The decrease could be corrected by the addition of cytochrome c in vitro. The phosphorylative activity of mitochondria remained unaffected. The decrease in oxidative rate was not observed when starving animals were given the anti-hypercholesterolaemic drug clofibrate. The total cellular concentration of cytochrome c was not affected by starvation. However, the concentration of the pigment in hepatic mitochondria isolated from starving animals was less than half that in normal mitochondria. Clofibrate-treated animals did not show a decreased concentration of cytochrome c in hepatic mitochondria. Mitochondria isolated from starving animals, though deficient in cytochrome c, did not show any decrease in succinate dehydrogenase activity or in the rate of substrate-dependent reduction of potassium ferricyanide or attendant phosphorylation. In coupled mitochondria, ferricyanide may not accept electrons from the cytochrome c in the respiratory chain. Starvation decreases the concentration of high-affinity binding sites for cytochrome c on the mitochondrial membrane. The dissociation constant increases in magnitude.

Item Type:	Journal Article
Publication:	Biochemical Journal
Publisher:	The Biochemical Society
Additional Information:	Copyright of this article belongs to the Biochemical Society.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	21 Aug 2009 11:37
Last Modified:	19 Sep 2010 05:38
URI:	http://eprints.iisc.ac.in/id/eprint/21563

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