Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings

Srivenugopal, KS and Adiga, PR (1980) Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings. In: Bochemical Journal, 190 (2). pp. 461-464.

PDF fulltext.pdf - Published Version Restricted to Registered users only Download (663kB) | Request a copy

Abstract

An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.

Item Type:	Journal Article
Publication:	Bochemical Journal
Publisher:	The Biochemical Society
Additional Information:	Copyright of this article belongs to the Biochemical Society.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Jul 2009 06:55
Last Modified:	01 Mar 2012 06:37
URI:	http://eprints.iisc.ac.in/id/eprint/21263

Actions (login required)

View Item