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Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings

Srivenugopal, KS and Adiga, PR (1980) Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings. In: Bochemical Journal, 190 (2). pp. 461-464.

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An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.

Item Type: Journal Article
Publication: Bochemical Journal
Publisher: The Biochemical Society
Additional Information: Copyright of this article belongs to the Biochemical Society.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 17 Jul 2009 06:55
Last Modified: 01 Mar 2012 06:37
URI: http://eprints.iisc.ac.in/id/eprint/21263

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