Ammonium ions prevent methylation of uridine to ribothymidine in Azotobacter vinelandii tRNA

Ajitkumar, P and Cherayil, Joseph D (1986) Ammonium ions prevent methylation of uridine to ribothymidine in Azotobacter vinelandii tRNA. In: Journal of Biosciences, 10 (2). pp. 267-276.

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Abstract

It was shown that tRNA from Azotobacter vinelandii grown in the presence of ammonium chloride lacks ribothymidine while that grown in the absence of the ammonium salt contains this modified nucleoside. [32P]-Labelled tRNA from this organism grown in a medium containing the ammonium salt was digested with RNase T1 and the pseudouridinecontaining tetranucleotide, common to all tRNAs was isolated and analysed for the nucleoside replacing the ribothymidine. It was found to be uridine. Cells previously labelled with [32P]- phosphate in the ammonium salt medium were washed and incubated in the ammonium saltfree medium to test whether ribothymidine would be formed upon removal of the ammoniumions. Methylation of the uridine did not take place.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Ribothymidine;uridine methylation;tRNA modification; Azotobacter vinelandii tRNA.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	01 Jul 2009 09:36
Last Modified:	19 Sep 2010 05:36
URI:	http://eprints.iisc.ac.in/id/eprint/21171

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