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The poly dA helix: a new structural motif for high performance DNA-based molecular switches

Chakraborty, Saikat and Sharma, Suruchi and Maiti, Prabal K and Krishnan, Yamuna (2009) The poly dA helix: a new structural motif for high performance DNA-based molecular switches. In: Nucleic acids research, 37 (9). pp. 2810-2817.

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We report a pH-dependent conformational transition in short, defined homopolymeric deoxyadenosines (dA(15)) from a single helical structure with stacked nucleobases at neutral pH to a double-helical, parallel-stranded duplex held together by AH-HA base pairs at acidic pH. Using native PAGE, 2D NMR, circular dichroism (CD) and fluorescence spectroscopy, we have characterized the two different pH dependent forms of dA(15). The pH-triggered transition between the two defined helical forms of dA(15) is characterized by CD and fluorescence. The kinetics of this conformational switch is found to occur on a millisecond time scale. This robust, highly reversible, pH-induced transition between the two well-defined structured states of dA(15)represents a new molecular building block for the construction of quick-response, pH-switchable architectures in structural DNA nanotechnology.

Item Type: Journal Article
Publication: Nucleic acids research
Publisher: Oxford university press
Additional Information: Copyright for this article belongs to Oxford university press.
Department/Centre: Division of Physical & Mathematical Sciences > Physics
Date Deposited: 11 Dec 2009 10:50
Last Modified: 19 Sep 2010 05:34
URI: http://eprints.iisc.ac.in/id/eprint/20838

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