Crystalline A-DNA: The X-Ray Analysis of the Fragment d(G-G-T-A-T-A-C-C)

Zippora, Shakkedt and Cruse, WBT and Rabinovicht, D and Olga, Kennards and Egertt, E and Salisburyt, SA and Graciela, Salai and Viswamitrata, MA (1981) Crystalline A-DNA: The X-Ray Analysis of the Fragment d(G-G-T-A-T-A-C-C). In: Proceedings of the Royal Society of London. Series B, Biological Sciences,, 213 (1193). pp. 479-487.

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Abstract

An A-DNA type double helical conformation was observed in the single crystal X-ray structure of the octamer d(G-G-T-A-T-A-C-C), 1, and its 5-bromouracil-containing analogue, 2. The structure of the isomorphous crystals (space group P61) was solved by a search technique based on packing criteria and R-factor calculations, with use of only low order data. At the present stage of refinement the R factors are 31 % for 1 and 28 % for 2 at a resolution of 2.25 A (0.225 nm). The molecules interact through their minor grooves by hydrogen bonding and base to sugar van der Waals contacts. The stable A conformation observed in the crystal may have some structural relevance to promoter regions where the T-A-T-A sequence is frequently found.

Item Type:	Journal Article
Publication:	Proceedings of the Royal Society of London. Series B, Biological Sciences,
Publisher:	The Royal Society London
Additional Information:	Copyright of this article belongs to The Royal Society London
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	30 May 2009 06:36
Last Modified:	01 Mar 2019 10:20
URI:	http://eprints.iisc.ac.in/id/eprint/20131

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