The effects of cholesterol inclusion on the vesicular membranes of cationic lipids

Bhattacharya, Santanu and Haldar, Saubhik (1996) The effects of cholesterol inclusion on the vesicular membranes of cationic lipids. In: Biochimica et Biophysica Acta, 1283 (01). pp. 21-30.

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Abstract

Small unilamellar vesicles formed from four cationic lipids in the absence and the presence of varying amounts of cholesterol were studied using fluorescence polarization and H-1-NMR techniques. The fluorescence polarization data clearly indicate that the packing order in the cationic lipid bilayers are affected by inclusion of cholesterol. importantly, this effect exists also with a cationic lipid that is devoid of any formal linkage region where the interaction of the lipid with cholesterol through hydrogen bonding is not feasible. The interactions of cholesterol with different types of cationic lipids in excess water have also been examined in multilamellar dispersions using proton magnetic resonance spectroscopy. In all the cases, the methylene proton linewidths in the NMR spectra respond to the addition of cholesterol to vesicles. Hydrophobic association of the lipid and cholesterol imposes restriction on the chain (CH2)(n) motions, leaving the terminal CH3 groups relatively mobile. On the basis of energy-minimized structural models, a rationale of the cholesterol-cationic lipid assembly has also been presented.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Membrane vesicle;Cationic lipid;Headgroup-chain linkage; Cholesterol;Fluorescence anisotropy;Phase transmon.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	02 Jun 2009 10:40
Last Modified:	19 Sep 2010 05:30
URI:	http://eprints.iisc.ac.in/id/eprint/19759

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