Heat shock protein 70 on Neuro2a cells is a putative receptor for Japanese encephalitis virus

Das, Soma and Laxminarayana, Suhas Venkataramana and Chandra, Nagasuma and Ravi, Vasanthapuram and Desai, Anita (2009) Heat shock protein 70 on Neuro2a cells is a putative receptor for Japanese encephalitis virus. In: Virology, 385 (1). pp. 47-57.

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Abstract

Japanese encephalitis virus (JEV) envelope (E) protein has been shown to play a critical role in attachment to cells. However, the receptor interacting with envelope protein has not been conclusively identified. Using mouse neuroblastoma (Neuro2a) cells and purified JEV-E protein in `Virus Overlay Protein Binding Assay' followed by MALDI-TOF analysis, we identified `heat shock protein 70' (Hsp70) as a possible receptor for JEV. Indirect immunofluorescence and flow-cytometry analysis demonstrated localization of Hsp70 on Neuro2a cell surface. Co-immunoprecipitation followed by Western blot analysis reconfirmed the interaction between Hsp70 and JEV-E protein. Further, anti-Hsp70 polyclonal-antibodies were able to block JEV entry into Neuro2a cells. Additionally, using the bioinformatic tool - FTDOCK, clocking between the proteins was performed. Amongst six interacting structural poses studied one pose involving RGD motif on JEV-E and leucine(539) on Hsp70 displayed stable interaction. These observations indicate that Hsp70 serves as putative receptor for JEV in Neuro2A cells.

Item Type:	Journal Article
Publication:	Virology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	JEV;Receptor;Hsp70;Neuro2a cells.
Department/Centre:	Division of Information Sciences (Doesn't exist now) > BioInformatics Centre
Date Deposited:	23 Jul 2009 10:53
Last Modified:	19 Sep 2010 05:29
URI:	http://eprints.iisc.ac.in/id/eprint/19666

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