Guanylyl Cyclase C Receptor: Regulation of Catalytic Activity by ATP

Bhandari, Rashna and Suguna, K and Visweswariah, Sandhya S (1999) Guanylyl Cyclase C Receptor: Regulation of Catalytic Activity by ATP. In: Bioscience Reports, 19 (3). pp. 179-188.

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Abstract

Guanylyl cyclase C (GCC), a member of the family of membrane bound guanylyl cyclases is the receptor for the heat-stable enterotoxin (ST) peptides and the guanylin family of endogenous peptides. GCC is activated upon ligand binding to increase intracellular cGMP levels, which in turn activates other downstream signalling events in the cell. GCC is also activated in vitro by nonionic detergents. We have used the T84 cell line as a model system to investigate the regulation of GCC activity by ATP. Ligand-stimulated GCC activity is potentiated in the presence of ATP, whereas detergent-stimulated activity is inhibited. The potentiation of GCC activity by ATP is dependent on the presence of Mg2+ ions, and is probably brought about by a direct binding of Mg-ATP to GCC. The protein kinase-like domain of GCC, which has earlier been shown to play a critical role in the regulation of GCC activity, may be a possible site for the binding of Mg-ATP to GCC.

Item Type:	Journal Article
Publication:	Bioscience Reports
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Guanylyl Cyclase C;Protein kinases;cGMP.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	11 Aug 2009 10:39
Last Modified:	19 Sep 2010 05:29
URI:	http://eprints.iisc.ac.in/id/eprint/19598

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