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X-Ray Studies On The Bilayer Structure Of Trypsin-Treated Rat-Brain Myelin

Ramakumar, Suryanarayanarao and Viswamitra, MA and Mohan, PM and Sastry, PS (1995) X-Ray Studies On The Bilayer Structure Of Trypsin-Treated Rat-Brain Myelin. In: Multidisciplinary Interaction Meeting on Neurosciences,Indian Inst Sci, Dept Phys, Bangalore, India, APR 16-18, 1993, Bangalore, India.

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Trypsin-treated rat brain myelin was subjected to biochemical and X-ray studies. Untreated myelin gave rise to a pattern of three rings with a fundamental repeat period of 155 Angstrom consisting of two bilayers per repeat period, whereas myelin treated with trypsin showed a fundamental repeat period of 75 Angstrom with one bilayer per repeat period. The integrated raw intensity of the h=4 reflection with respect to the h=2 reflection is 0.38 for untreated myelin. The corresponding value reduced to 0.23, 0.18, 0.17 for myelin treated with 5, 10, 40 units of trypsin per mg of myelin, respectively, for 30 min at 30 degrees C. The decrease in relative raw intensity of the higher-order reflection relative to the lower-order reflection is suggestive of a disordering of the phosphate groups upon trypsin treatment or an increased mosaicity of the membrane or a combination of both these effects, However, trypsin treatment does not lead to a complete breakdown of the membrane, The integrated intensity of the h=1 reflection, though weak, is above the measurable threshold for untreated myelin, whereas the corresponding intensity is below the measurable threshold for trypsin-treated myelin, indicating a possible asymmetric to symmetric transition of the myelin bilayer structure about its centre after trypsin treatment.

Item Type: Conference Paper
Publisher: Indian Academy of Sciences
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: Multidisciplinary Sciences
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 19 Feb 2010 05:48
Last Modified: 19 Sep 2010 05:27
URI: http://eprints.iisc.ac.in/id/eprint/19228

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