Subramanian, N and Subramanian, Sarada and Adiga, Radhakantha P (1996) Antigenic similarities among biotin-binding proteins. In: Biochemistry and Molecular Biology International, 40 (04). pp. 663-670.
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Abstract
Using polyclonal antisera to avidin, streptavidin, and biotin binding protein-II (BBP-II), the extents of immunological cross-reactivities shared by this family of biotin-binders were investigated. In Western blot analysis, varying degrees of cross-reactivities among these proteins depending on the antisera used was observed. Using liquid phase radioimmunoassay, evidence for the existence of common antigenic determinants shared by all the biotin-binders was deduced. Antibodies directed against such epitopes inhibited the binding of [H-3]biotin to as well as displace the bound ligand from all the three high affinity biotin-binders in a dose dependent manner
Item Type: | Journal Article |
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Publication: | Biochemistry and Molecular Biology International |
Publisher: | Academic Press |
Additional Information: | Copyright of this article belongs to Academic Press. |
Keywords: | Biotin-binders;antibodies;ligand-binding;inhibition. |
Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
Date Deposited: | 26 Feb 2009 07:18 |
Last Modified: | 01 Mar 2012 07:13 |
URI: | http://eprints.iisc.ac.in/id/eprint/19080 |
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