Srinivasan, L and Gopinathan, KP (2001) Differential expression of individual gene copies from within a trna multigene family in the mulberry silkworm bombyx mori. In: Insect Molecular Biology, 10 (6). pp. 523-530.
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In mulberry silkworm Bombyx mori, tRNA(1)(Gly) constitutes a multigene family from which the individual members are transcribed at different levels in vitro in homologous nuclear extracts. We report here the quantification of functional transcripts of these gene copies in vivo In B. mori-derived BmN cells based on a suppression assay. The gene copies were converted to encode suppressor tRNAs and co-transfected into cell lines with reporter gene(s) harbouring one or more nonsense mutations and the reporter gene activity was quantified. Individual members of the gene family were transcribed to very high-, medium- and very low-levels, following the same pattern as in vitro. All these gene copies were maximally expressed in Bm cells as compared to other insect cell lines.
| Item Type: | Journal Article |
|---|---|
| Publication: | Insect Molecular Biology |
| Publisher: | John Wiley and Sons |
| Additional Information: | Copyright for this article belongs to John Wiley and Sons. |
| Keywords: | Bombyx mori;RNA polymerase III;Transcriptional Regulation;pol III transcription;tRNA genes |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 10 Sep 2004 |
| Last Modified: | 13 Jan 2012 08:41 |
| URI: | http://eprints.iisc.ac.in/id/eprint/1906 |
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