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Understanding protein–protein interactions by genetic suppression

Sujatha, Sitaraman and Chatterji, Dipankar (2000) Understanding protein–protein interactions by genetic suppression. In: Journal of Genetics, 79 (3). pp. 125-129.


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Protein-protein interactions influence many cellular processes and it is increasingly being felt that even a weak and remote interplay between two subunits of a protein or between two proteins in a complex may govern the fate of a particular biochemical pathway. In a bacterial system where the complete genome sequence is available, it is an arduous task to assign function to a large number of proteins. It is possible that many of them are peripherally associated with a cellular event and it is very difficult to probe such interaction. However, mutations in the genes that encode such proteins (primary mutations) are useful in these studies. Isolation of a suppressor or a second-site mutation that restores the phenotype abolished by the primary mutation could be an elegant yet simple way to follow a set of interacting proteins. Such a reversion site need not necessarily be geometrically close to the primary mutation site.

Item Type: Journal Article
Publication: Journal of Genetics
Publisher: Indian Academy of Sciences
Additional Information: The copyright of this artilce belongs to Indian Academy of Science
Keywords: genetic suppression;protein–protein interaction;allele specificity;long-range interaction
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 24 Sep 2004
Last Modified: 19 Sep 2010 04:16
URI: http://eprints.iisc.ac.in/id/eprint/1885

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