Determination of kinetic parameters and thermodynamic constant of antigen-antibody reaction by solid phase binding method

Murthy, GS (1997) Determination of kinetic parameters and thermodynamic constant of antigen-antibody reaction by solid phase binding method. In: Current Science, 73 (12). pp. 1097-1103.

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Abstract

Study of the dissociation of [I-125]hCG from immobilized MAb-[I-125]hCG complex was carried out. The dissociation data fitted into a two-step reaction mechanism of the interaction. Kinetic parameters obtained were consistent, and were used to obtain the thermodynamic constants of the interaction. The first step (epitope recognition reaction) was shown to be a high-affinity intermolecular binding reaction involving epitope-paratope interaction, while the transformation reaction (second step) is an intramolecular rearrangement of the [I-125]hCG-MAb complex formed in the first step. The method described has the potential to be automated.

Item Type:	Journal Article
Publication:	Current Science
Publisher:	Indian Academy Of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy Of Sciences.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	01 Jun 2009 05:35
Last Modified:	19 Sep 2010 05:25
URI:	http://eprints.iisc.ac.in/id/eprint/18844

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