In vitro processing of cucumber chloroplast tRNA Leu(CAA) precursor in a pea chloroplast soluble extract

Hande, Shailaja and Jayabaskaran, C (1998) In vitro processing of cucumber chloroplast tRNA Leu(CAA) precursor in a pea chloroplast soluble extract. In: Biochemistry and Molecular Biology International, 44 (02). pp. 417-424.

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Abstract

To study the sequential steps in the processing pathway of the chloroplast monocistronic intronless tRNA precursors, we examined cucumber chloroplast tRNA(Leu)(CAA) processing in a cucumber or pea chloroplast soluble extract. The tRNA(Leu)(CAA) precursor synthesized from SP6 RNA polymerase-directed transcription system, was used as a substrate. Incubation of the tRNA precursor with the pea extract resulted in processing of tRNA(Leu)(CAA) via 5'- and 3'-endonucleolytic cleavages followed by final trimming of extra 3' nucleotides by 3' exonuclease(s). No preferred order for endonucleolytic cleavages has been observed during the in vitro tRNA(Leu) processing and the simultaneous occurrence of the intermediates consisting of leader + tRNA(Leu) and tRNA(Leu) + trailer, indicate that either 5'- or 3'-endonucleolitic cleavage can occur as the first step in vitro.

Item Type:	Journal Article
Publication:	Biochemistry and Molecular Biology International
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	21 Aug 2009 05:17
Last Modified:	19 Sep 2010 05:25
URI:	http://eprints.iisc.ac.in/id/eprint/18775

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