Interaction of Co, Mn, Mg and Al with d(GCCCATGGC) and d(CCGGGCCCGG): a spectroscopic study

Champion, CS and Kumar, D and Rajan, MT and Rao, Jagannatha KS and Viswamitra, MA (1998) Interaction of Co, Mn, Mg and Al with d(GCCCATGGC) and d(CCGGGCCCGG): a spectroscopic study. In: Cellular and Molecular Life Sciences (CMLS), 54 (05). pp. 488-496.

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Abstract

Spectroscopic study of the interactions of metal ions, Co, Mn, Mg and Al with d(GCCCATGGGC) and d(CCGGGCCCGG) revealed the following. Metal ions Mn, Al and Mg at the lowest concentrations enhanced the t(m) of oligomers, whereas Mn and Mg at higher concentrations decreased the t(m). Co enhanced the t(m) of oligomers at higher concentrations. The studies have also indicated that Mn at lower concentrations displaced EtBr fluorescence, Mg and Co at moderate concentrations and Al only at higher concentrations. Addition of Co, Mn, Mg and Al altered the bands of the circulars dichroism (CD) spectra of the oligomers in a concentration-dependent manner. The CD spectra of d(GCCCATGGGC) and d(CCGGGCCCGG) indicated B and Z forms of DNA, respectively, in contrast to the A form observed in the crystal structures. Mg and Co at different ionic strength induced Z-B transition in d(CCGGGCCCGG), while Al at higher concentrations induced a Z-A transition. Mn did not induce any transition. This is the first report to show that Al causes structural transitions in sequence specific oligomers and has strong binding ability with CC-rich euchromatin oligomers.

Item Type:	Journal Article
Publication:	Cellular and Molecular Life Sciences (CMLS)
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	DNA;interactions;transitions;spectroscopy;trace elements.
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	25 Aug 2009 12:18
Last Modified:	19 Sep 2010 05:25
URI:	http://eprints.iisc.ac.in/id/eprint/18748

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