Immunological screening of a genomic M-bovis BCG library expressed in M-smegmatis and identification of the M-bovis BCG analog of ClpB

Bona, M and Nayak, R and Wu, M and Mincek, M and Ellner, JJ (1998) Immunological screening of a genomic M-bovis BCG library expressed in M-smegmatis and identification of the M-bovis BCG analog of ClpB. In: Tubercle and Lung Disease, 78 (02). pp. 133-141.

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Abstract

This work describes the screening of a M. bovis BCG cosmid library in M. smegmatis with a hyperimmune rabbit anti-BCG serum. Cross-reactive antibodies interfere with the detection of BCG specific antigens in M. smegmatis culture filtrates. We, therefore, screened parallel western blots with serum adsorbed with a M. smegmatis cell lysate and unadsorbed serum. Comparison of the western blots allowed distinction between BCG specific and cross-reactive M. smegmatis antigens. Thirty-one cosmids expressed BCG specific antigens. One of them, a hitherto undescribed 100 kDa antigen was subcloned, sequenced and expressed in E. coli. It shows a high degree of homology to ClpB, a member of the Clp family of proteases and was immunologically reactive with the rabbit hyperimmune serum against M. bovis BCG. A positive signal was also obtained with sera of patients with tuberculosis. This antigen is a previously unrecognized target of the human immune response to mycobacteria.

Item Type:	Journal Article
Publication:	Tubercle and Lung Disease
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	17 Aug 2009 06:10
Last Modified:	19 Sep 2010 05:25
URI:	http://eprints.iisc.ac.in/id/eprint/18721

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