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Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer

Johnson, Tanya and Ouhtit, Allal and Gaur, Rajiv and Fernando, Augusta and Schwarzenberger, Paul and Su, Joseph and Ismail, Mohamed F and El-Sayyad, Hassan I and Karande, Anjali and Elmageed, Zakaria Abd and Rao, Prakash and Raj, Madhwa (2009) Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer. In: Frontiers in Bioscience, 14 . pp. 3634-3640.

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Riboflavin carrier protein (RCP) is a growth- and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was over-expressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.

Item Type: Journal Article
Publication: Frontiers in Bioscience
Publisher: Frontiers in Bioscience
Additional Information: Copyright of this article belongs to Frontiers in Bioscience.
Keywords: Prostate Cancer;Riboflavin Carrier Protein;Tumor Marker; Antibody.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 04 May 2009 11:01
Last Modified: 04 May 2009 11:01
URI: http://eprints.iisc.ac.in/id/eprint/18614

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