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Effect of long-term treatment with aromatase inhibitor on testicular function of adult male bonnet monkeys (M-radiata)

Shetty, Gunapala and Krishnamurthy, Hanumanthappa and Krishnamurthy, Hegganahalli N and Bhatnagar, Ajay S and Moudgal, Nuggehali R (1998) Effect of long-term treatment with aromatase inhibitor on testicular function of adult male bonnet monkeys (M-radiata). In: Steroids, 63 (7-8). pp. 414-420.


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The role/need for estrogen in regulating testicular function of adult male bonnet monkeys (M. radiata) has been investigated by dosing orally a group of five normal males 2.5 mgs of CGP 47645, a long-acting nonsteroidal aromatase inhibitor (AI), once every 5 days for over 150 days. Such treatment resulted in a 10-fold increment in nocturnal serum testosterone (T) levels, which were sustained for 85 days of treatment, and a twofold increment in basal serum T levels was present throughout the 150 days of treatment. Analysis of ejaculated semen showed a marked reduction (similar to 90%) in sperm counts in four out of five monkeys between Days 55-85 of treatment. During this period, the motility scare also was markedly reduced from a normal score of 3-5 to 0-2. Flow cytometric analysis of testicular germ cells obtained from biopsy tissue taken on Days 63 and 120 indicated a marked reduction only in elongating/elongated spermatid population (compared to Day 0 values), suggesting inhibition in spermiogenic process. Epididymal sperm maturation also seemed effected as sperm chromatin, on flow cytometric analysis for decondensability following exposure to 5 mM dithiotreitol, showed to be in a hypercondensed state. This study thus indicates that estrogen has an important role in providing normal testicular and sperm function in the primate

Item Type: Journal Article
Publication: Steroids
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: estrogen;primate;spermatogenesis;testicular function.
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 20 May 2009 08:31
Last Modified: 19 Sep 2010 05:02
URI: http://eprints.iisc.ac.in/id/eprint/18467

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