The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli

Thanedar, Swapna and Dineshkumar, TK and Varshney, Umesh (2001) The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli. In: Journal of Bacteriology, 183 (24). pp. 7397-7402.

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Abstract

Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show, by using trmA strains of Escherichia coli (defective for rT modification) and a sensitive in vivo initiation assay system, that the lack of rT modification in the initiators is not sufficient to effect formylation- independent initiation of protein synthesis.

Item Type:	Journal Article
Publication:	Journal of Bacteriology
Publisher:	American Society for Microbiology
Additional Information:	The copyright of this article belongs to American Society for Microbiology.
Keywords:	Formylmethionine transfer-rna;Protein synthesis;Streptococcus-faecalis;Recognition;Methionine;Stem
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	09 Sep 2004
Last Modified:	19 Sep 2010 04:16
URI:	http://eprints.iisc.ac.in/id/eprint/1843

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