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Deprivation of FSH support affects chromatin status and acrosomal glycoprotein concentration of sperm of monkey and man

Krishnamurthy, H and Kumar, KMP and Moudgal, NR and Sairam, MR (1999) Deprivation of FSH support affects chromatin status and acrosomal glycoprotein concentration of sperm of monkey and man. In: Biology of Reproduction, 60 . p. 165.

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Chromatin packaging and acrosomal glycoprotein concentration (PSA-FITC binding) of sperm voided by normal, healthy proven fertile volunteer men as well as bonnet monkeys, both actively immunized with oFSH, were examined at different times of study by flow cytometry. Compared to day 0 values PSA-FITC binding to acrosome of sperm of both monkey and man was significantly low during periods when cross-reactive FSH antibody titre was high and endogenous FSH was not measurable. Similarly, dithiothreitol (DTT) induced decondensation of sperm DNA as measured by ethidium bromide binding was significantly high compared to day 0 values in both man & monkey. Binding of DTT exposed sperm DNA to Mithramycin, a specific indicator of chromatin packaging, was also significantly high in both man and monkey following FSH immunization. Quantification of relative red and green fluorescence of acridine orange following its binding to acid denatured sperm DNA results in the t values, index of chromatin packaging to significant increase following FSH immunization. Blockade of FSH function in monkeys by active immunization with a recombinant oFSH receptor protein fragment also resulted in production of sperm exhibiting similar defects in chromatin packaging and reduced PSA-FITC binding. Thus both monkey and man appear to respond to lack of FSH signaling by producing defective sperm whose characteristics are similar to that exhibited by sperm of some class of infertile men.

Item Type: Journal Article
Publication: Biology of Reproduction
Publisher: Society for the Study of Reproduction
Additional Information: Copyright of this article belongs to Society for the Study of Reproduction.
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 30 May 2009 17:42
Last Modified: 30 May 2009 17:42
URI: http://eprints.iisc.ac.in/id/eprint/18328

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