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Analysis of gene expression during embryonic development in mulberry silkworm Bombyx mori

Singh, A and Gopinathan, Karumathil P (1997) Analysis of gene expression during embryonic development in mulberry silkworm Bombyx mori. In: Current Science, 72 (3). pp. 214-218.

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Official URL: http://www.ias.ac.in/j_archive/currsci/72/3/214-21...

Abstract

We have developed a method for dechorionation and devitellinization of the silkworm eggs without damage, to facilitate the analysis of gene expression during embryonic development of Bombyx mori. Making use of antibodies available from heterologous systems, the spatio-temporal expression patterns of peroxidase and proliferating cell nuclear antigen have been directly visualized in whole mount embryos at various stages of development without the need for generating transformed lines carrying specific reporter constructs. The B. mori system, previously unamenable for such studies, could thus serve as an attractive model for molecular analysis of insect development.

Item Type: Journal Article
Publication: Current Science
Publisher: Indian Academy of Sciences
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 22 Apr 2009 13:08
Last Modified: 19 Sep 2010 05:01
URI: http://eprints.iisc.ac.in/id/eprint/18316

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