Cloning and expression of an Acyl-CoA dehydrogenase from Mycobacterium tuberculosis

Mahadevan, Usha and Padmanaban, G (1998) Cloning and expression of an Acyl-CoA dehydrogenase from Mycobacterium tuberculosis. In: Biochemical and Biophysical Research Communications, 244 (3). pp. 893-897.

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Abstract

A gene hom Mycobacterium tuberculosis coding for acyl-CoA dehydrogenase was cloned, overexpressed and characterized on the basis of enzyme activity with various chain length substrates, The results show that the protein is a medium chain acyl-CoA dehydrogenase (MCADH). The mycobacterium protein expressed appears to be unique, since by comparison, the active site glutamic acid of the protein does not lie in the same position as other well characterized MCADH, but in a position present in long chain and isovaleryl acyl-CoA dehydrogenases (LCADH and IVDH). (C) 1998 Academic Press.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	13 Mar 2009 07:17
Last Modified:	19 Sep 2010 05:00
URI:	http://eprints.iisc.ac.in/id/eprint/18256

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