Calcium levels correlate with cell cycle phase and affect the level of the cyclin B transcript in Dictyostelium discoideum

Azhar, M and Kreff, M and Saran, S and Weeks, G and Nanjundiah, Vidyanand (1998) Calcium levels correlate with cell cycle phase and affect the level of the cyclin B transcript in Dictyostelium discoideum. In: FEMS Microbiology Letters, 161 (1). pp. 193-199.

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Abstract

In pre-aggregation amoebae of Dictyostelium discoideum, phenotypic differences with respect to cellular Ca2+ and cell cycle phases are known to bias post-aggregative cell-type choice. Using chlortetracycline fluorescence as an indicator, we found that cellular Ca2+ is highest at the S phase of the cell cycle. Upon increasing the level of Ca2+ with the help of the calcium ionophore A23187, there is a significant decrease in the cyclin B (clb1) mRNA level; the cdc2 mRNA level shows a marginal decrease. These results suggest that the effect of Ca2+ and the cell cycle on cell fate could be exerted at the level of transcription, or message stability, of specific genes

Item Type:	Journal Article
Publication:	FEMS Microbiology Letters
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Cell cycle;calcium;cyclin B;Dictyostelium.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	05 Mar 2009 06:34
Last Modified:	19 Sep 2010 05:00
URI:	http://eprints.iisc.ac.in/id/eprint/18156

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