Agrobacterium-mediated genetic transformation in hot chilli (Capsicum annuum L. var. Pusa jwala)

Manoharan, M and Sree Vidya, CS and Lakshmi Sita, G (1998) Agrobacterium-mediated genetic transformation in hot chilli (Capsicum annuum L. var. Pusa jwala). In: Plant Science, 131 (1). pp. 77-83.

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Abstract

A protocol for regeneration and genetic transformation has been established for chilli (Capsicum annuum L. var. Pusa jwala). High frequency regeneration of shoot buds from cotyledonary leaves was achieved with Murashige and Skoog's (MS) medium supplemented with 0.5 mg/l thidiazuron. Elongation of shoot buds and subsequent rooting was obtained on half-strength MS medium with 0.5 mg/l IAA. The Agrobacterium tumefaciens strain EHA 105 carrying a binary vector plasmid pBI 121 has been used for transformation. The cotyledonary explants from in vitro grown shoots were cocultivated for 2 days. Shoot buds were produced on the regeneration medium containing kanamycin (50 mg/l) and cefotaxime (400 mg/l). The shoot buds were elongated and rooted in the presence of kanamycin (25 mg/l). The transgenic nature of the regenerated plants were confirmed by the histochemical staining of GUS, polymerase chain reaction (PCR) and Southern hybridization analyses of nptII gene.

Item Type:	Journal Article
Publication:	Plant Science
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	regeneration;thidiazuron (TDZ);genetic transformation; beta-glucuronidase (GUS);neomycin phosphotransferase (NPT II);chilli (Capsicum annuum L).
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	16 Feb 2009 11:20
Last Modified:	19 Sep 2010 05:00
URI:	http://eprints.iisc.ac.in/id/eprint/18104

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