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Antiidiotypic antibody to gonadotropin releasing hormone as probe for cell surface receptors

Karande, Anjali A and Rajeshwari, Krishnamurthy and Padmanaban, Deepa and Ravichandra, Balijepalli and Joshi, Preeti G and Yadav, Poonam and Shobha, Prahlad (1998) Antiidiotypic antibody to gonadotropin releasing hormone as probe for cell surface receptors. In: Biochemistry and Molecular Biology International, 46 (3). pp. 449-459.

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Monoclonal antibodies were raised to the idiotype of a gonadotropin releasing hormone (GnRH) specific antibody, one of which was found to bind specifically to GnRH receptors present on pituitary gonadotrophs, placental syncytiotrophoblasts and testicular Leydig cells. These observations were confirmed by Western and ligand blotting as well as by the ability of the antibody to induce an increase in intracellular Ca++ in a mouse gonadotroph cell-line viz., alpha T3-1.

Item Type: Journal Article
Publication: Biochemistry and Molecular Biology International
Publisher: Academic Press
Additional Information: Copyright of this article belongs to Academic Press.
Keywords: GnRH receptor;antiidiotypic monoclonal antibody; immunohistochemistry;Western blotting;intracellular calcium.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Feb 2009 11:52
Last Modified: 15 Jan 2013 06:59
URI: http://eprints.iisc.ac.in/id/eprint/18054

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