Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: Protection against oxidative damage to DNA

Bhat, VB and Madyastha, KM (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: Protection against oxidative damage to DNA. In: Biochemical And Biophysical Research Communications, 285 (2). pp. 262-266.

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Abstract

Peroxynitrite (ONOO-) is known to inactivate important cellular targets and also mediate oxidative damage in DNA, The present study has demonstrated that phycocyanin, a biliprotein from spirulina platensis and its chromophore, phycocyanobilin (PCB), efficiently scavenge ONOO-, a potent physiological inorganic toxin, Scavenging of ONOO- by phycocyanin and PCB was established by studying their interaction with ONOO- and quantified by using competition kinetics of pyrogallol red bleaching assay. The relative antioxidant ratio and IC50 value clearly indicate that phycocyanin is a more efficient ONOO- scavenger than PCB. The present study has also shown that PCB significantly inhibits the ONOO--mediated single-strand breaks in supercoiled plasmid DNA in a dose-dependent manner with an IC50 value of 2.9 +/- 0.6 muM. These results suggest that phycocyanin, has the ability to inhibit the ONOO--mediated deleterious biological effects and hence has the potential to be used as a therapeutic agent.

Item Type:	Journal Article
Publication:	Biochemical And Biophysical Research Communications
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Keywords:	Phycocyanin;phycocyanobilin;peroxynitrite scavenging;pyrogallol red;DNA damage;antioxidant
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	10 Feb 2010 08:30
Last Modified:	19 Sep 2010 04:56
URI:	http://eprints.iisc.ac.in/id/eprint/17368

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